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hepg2 with dhcr24 deletion cells  (Synthego Inc)

 
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    Structured Review

    Synthego Inc hepg2 with dhcr24 deletion cells
    Representative chromatograms of sterols isolated from native <t>HepG2</t> cells ( a , b ) and HepG2 DHCR KO cells ( c , d ). Each chromatogram is presented in two magnifications to account for a higher quantity of cholesterol compared to intermediate sterols ( a , b ) or desmosterol and cholesterol compared to other sterols ( c , d ). Zymosterol (A), 24-dehydrolathosterol (B), 7-dehydrodesmosterol (C), 7-desmosterol (D), zymostenol (E), F-lathosterol (F), lathosterol-d7 (G), TMAS (I), holesterol (J), lanosterol (K), dihydrolanosterol (L), and an unknown peak with the MRM of 365/199 (X). All peaks with the same MRM transition (A, B, D and E, F, J) are chromatographically separated. Peak of cholesterol is depicted for illustrative purpose only.
    Hepg2 With Dhcr24 Deletion Cells, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepg2+with+dhcr24+deletion+cells/hepg2+pgam5+knockout+cell+line/pmc07571030-113-8-14
    Average 90 stars, based on 1 article reviews
    hepg2 with dhcr24 deletion cells - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Simplified LC-MS Method for Analysis of Sterols in Biological Samples"

    Article Title: Simplified LC-MS Method for Analysis of Sterols in Biological Samples

    Journal: Molecules

    doi: 10.3390/molecules25184116

    Representative chromatograms of sterols isolated from native HepG2 cells ( a , b ) and HepG2 DHCR KO cells ( c , d ). Each chromatogram is presented in two magnifications to account for a higher quantity of cholesterol compared to intermediate sterols ( a , b ) or desmosterol and cholesterol compared to other sterols ( c , d ). Zymosterol (A), 24-dehydrolathosterol (B), 7-dehydrodesmosterol (C), 7-desmosterol (D), zymostenol (E), F-lathosterol (F), lathosterol-d7 (G), TMAS (I), holesterol (J), lanosterol (K), dihydrolanosterol (L), and an unknown peak with the MRM of 365/199 (X). All peaks with the same MRM transition (A, B, D and E, F, J) are chromatographically separated. Peak of cholesterol is depicted for illustrative purpose only.
    Figure Legend Snippet: Representative chromatograms of sterols isolated from native HepG2 cells ( a , b ) and HepG2 DHCR KO cells ( c , d ). Each chromatogram is presented in two magnifications to account for a higher quantity of cholesterol compared to intermediate sterols ( a , b ) or desmosterol and cholesterol compared to other sterols ( c , d ). Zymosterol (A), 24-dehydrolathosterol (B), 7-dehydrodesmosterol (C), 7-desmosterol (D), zymostenol (E), F-lathosterol (F), lathosterol-d7 (G), TMAS (I), holesterol (J), lanosterol (K), dihydrolanosterol (L), and an unknown peak with the MRM of 365/199 (X). All peaks with the same MRM transition (A, B, D and E, F, J) are chromatographically separated. Peak of cholesterol is depicted for illustrative purpose only.

    Techniques Used: Isolation

    Concentrations of sterol intermediates isolated from HepG2 and HepG2 DHCR-KO cell lines. All concentrations are calculated as ng/mL per 10 7 , to normalize sterol concentration based on the number of counted cells. All data are represented as mean with SD, black dots represent individual measurements.
    Figure Legend Snippet: Concentrations of sterol intermediates isolated from HepG2 and HepG2 DHCR-KO cell lines. All concentrations are calculated as ng/mL per 10 7 , to normalize sterol concentration based on the number of counted cells. All data are represented as mean with SD, black dots represent individual measurements.

    Techniques Used: Isolation, Concentration Assay

    Concentration of sterols isolated from HepG2 and HepG2 DHCR24 KO cell cultures.
    Figure Legend Snippet: Concentration of sterols isolated from HepG2 and HepG2 DHCR24 KO cell cultures.

    Techniques Used: Concentration Assay, Isolation

    Related Articles

    Isolation:

    Article Title: Simplified LC-MS Method for Analysis of Sterols in Biological Samples
    Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM), Penicillin-Streptomycin (P/S), Trypsin, and Fetal Bovine Serum (FBS) were bought from Merck (Darmstadt, Germany). .. Here, 1 × 10 6 of HepG2 and HepG2 with DHCR24 deletion (ordered from Synthego, Menalo Park, CA, USA) cells were plated on a T75 culture flask for each sterol isolation. ..

    Article Title: Simplified LC-MS Method for Analysis of Sterols in Biological Samples
    Article Snippet: .. Isolation and Culture of Human Hepatocytes Here, 1 × 106 of HepG2 and HepG2 with DHCR24 deletion (ordered from Synthego, Menalo Park, CA, USA) cells were plated on a T75 culture flask for each sterol isolation. ..



    Similar Products

    hepg2 with dhcr24 deletion cells  (Synthego Inc)
    90
    Synthego Inc hepg2 with dhcr24 deletion cells
    Representative chromatograms of sterols isolated from native <t>HepG2</t> cells ( a , b ) and HepG2 DHCR KO cells ( c , d ). Each chromatogram is presented in two magnifications to account for a higher quantity of cholesterol compared to intermediate sterols ( a , b ) or desmosterol and cholesterol compared to other sterols ( c , d ). Zymosterol (A), 24-dehydrolathosterol (B), 7-dehydrodesmosterol (C), 7-desmosterol (D), zymostenol (E), F-lathosterol (F), lathosterol-d7 (G), TMAS (I), holesterol (J), lanosterol (K), dihydrolanosterol (L), and an unknown peak with the MRM of 365/199 (X). All peaks with the same MRM transition (A, B, D and E, F, J) are chromatographically separated. Peak of cholesterol is depicted for illustrative purpose only.
    Hepg2 With Dhcr24 Deletion Cells, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepg2+with+dhcr24+deletion+cells/hepg2+pgam5+knockout+cell+line/pmc07571030-113-8-14
    Average 90 stars, based on 1 article reviews
    hepg2 with dhcr24 deletion cells - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Representative chromatograms of sterols isolated from native HepG2 cells ( a , b ) and HepG2 DHCR KO cells ( c , d ). Each chromatogram is presented in two magnifications to account for a higher quantity of cholesterol compared to intermediate sterols ( a , b ) or desmosterol and cholesterol compared to other sterols ( c , d ). Zymosterol (A), 24-dehydrolathosterol (B), 7-dehydrodesmosterol (C), 7-desmosterol (D), zymostenol (E), F-lathosterol (F), lathosterol-d7 (G), TMAS (I), holesterol (J), lanosterol (K), dihydrolanosterol (L), and an unknown peak with the MRM of 365/199 (X). All peaks with the same MRM transition (A, B, D and E, F, J) are chromatographically separated. Peak of cholesterol is depicted for illustrative purpose only.

    Journal: Molecules

    Article Title: Simplified LC-MS Method for Analysis of Sterols in Biological Samples

    doi: 10.3390/molecules25184116

    Figure Lengend Snippet: Representative chromatograms of sterols isolated from native HepG2 cells ( a , b ) and HepG2 DHCR KO cells ( c , d ). Each chromatogram is presented in two magnifications to account for a higher quantity of cholesterol compared to intermediate sterols ( a , b ) or desmosterol and cholesterol compared to other sterols ( c , d ). Zymosterol (A), 24-dehydrolathosterol (B), 7-dehydrodesmosterol (C), 7-desmosterol (D), zymostenol (E), F-lathosterol (F), lathosterol-d7 (G), TMAS (I), holesterol (J), lanosterol (K), dihydrolanosterol (L), and an unknown peak with the MRM of 365/199 (X). All peaks with the same MRM transition (A, B, D and E, F, J) are chromatographically separated. Peak of cholesterol is depicted for illustrative purpose only.

    Article Snippet: Here, 1 × 10 6 of HepG2 and HepG2 with DHCR24 deletion (ordered from Synthego, Menalo Park, CA, USA) cells were plated on a T75 culture flask for each sterol isolation.

    Techniques: Isolation

    Concentrations of sterol intermediates isolated from HepG2 and HepG2 DHCR-KO cell lines. All concentrations are calculated as ng/mL per 10 7 , to normalize sterol concentration based on the number of counted cells. All data are represented as mean with SD, black dots represent individual measurements.

    Journal: Molecules

    Article Title: Simplified LC-MS Method for Analysis of Sterols in Biological Samples

    doi: 10.3390/molecules25184116

    Figure Lengend Snippet: Concentrations of sterol intermediates isolated from HepG2 and HepG2 DHCR-KO cell lines. All concentrations are calculated as ng/mL per 10 7 , to normalize sterol concentration based on the number of counted cells. All data are represented as mean with SD, black dots represent individual measurements.

    Article Snippet: Here, 1 × 10 6 of HepG2 and HepG2 with DHCR24 deletion (ordered from Synthego, Menalo Park, CA, USA) cells were plated on a T75 culture flask for each sterol isolation.

    Techniques: Isolation, Concentration Assay

    Concentration of sterols isolated from HepG2 and HepG2 DHCR24 KO cell cultures.

    Journal: Molecules

    Article Title: Simplified LC-MS Method for Analysis of Sterols in Biological Samples

    doi: 10.3390/molecules25184116

    Figure Lengend Snippet: Concentration of sterols isolated from HepG2 and HepG2 DHCR24 KO cell cultures.

    Article Snippet: Here, 1 × 10 6 of HepG2 and HepG2 with DHCR24 deletion (ordered from Synthego, Menalo Park, CA, USA) cells were plated on a T75 culture flask for each sterol isolation.

    Techniques: Concentration Assay, Isolation